The charges relate to an incident in Barlestone Road in April.
Henry Gaskin, 53, and Carol Gaskin, 51, both of Lands End Road, Doncaster, pleaded not guilty to the charge of attempted murder.
Carol Gaskin also pleaded not guilty to a charge of assisting an offender.
The charges relate to an incident on Thursday (29 April) when a man was found critically injured in Barlestone Road.
A trial has been set for 29th November, which will run for 10 days.
Both Henry and Carol have been released on condition bail, if they can provided £100,000 each in surety.
Bonfire Night: Firework safety advice for people celebrating across Leicestershire and Warwickshire
Thousands of poppies transform Loughborough ahead of Remembrance Sunday
Loughborough Remembrance Sunday parade and service
Loughborough Fair returns for four days of rides and family fun
Two hours of free parking at Loughborough car parks for Christmas
Man convicted after stabbing two men through car window in Quorn
Weekly food waste collections begin across Charnwood
Deadline approaching for Leicestershire secondary school applications
